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Updated: Apr 11, 2026

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage
Published on: April 23, 2017
Effect of passive ultrasonic irrigation on diffusion of hydroxyl ion through radicular dentine
Fernanda Helaine Cidade1, Tamer Ferreira Schmidt1, Luciane Geanini Pena Dos Santos1
1Postgraduate Program of the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Objective:
This study investigated the effect of passive ultrasonic irrigation (PUI) on diffusion of hydroxyl ions through radicular dentine.
Materials And Methods:
After chemomechanical preparation of root canals in 60 human teeth, the cementoenamel junction and the apical 3 mm of each root were covered with fast-setting adhesive. Four final irrigation protocols were applied (n = 10): group (G)1: irrigation with EDTA + NaOCl; G2: EDTA + PUI + NaOCl; G3: EDTA+(NaOCl + PUI); G4: (EDTA + PUI) + (NaOCl + PUI). Ten teeth irrigated with distilled water followed by PUI (G5) served as the negative control. After drying, the canals were filled with calcium hydroxide paste (CH), sealed and kept in individual vials containing 10 mL of distilled water with known pH values. At 7, 14, and 21 days, the pH of the water in the vials was measured. The pH values in various groups were analyzed with two-way ANOVA (irrigation protocol and time period as factors) and Holm-Sidak multiple comparison test (α = 0.05).
Results:
Changes in pH was not significantly different among groups (P = 0.651) but was significant different among different time periods (P < 0.0001). For all groups, ion diffusion was higher at 14 and 21 days than at 7 days.
Conclusions:
PUI has no effect on diffusion of hydroxyl ions through radicular dentine. When CH is used as temporary filling material, a waiting period of at least 14 days is required to create an alkaline environment within the radicular dentine.
Clinical Relevance:
The use of PUI during final irrigation phase does not improve the action of CH when it is used as temporary filling material.
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