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Updated: Apr 11, 2026

Whole Mount Labeling of Cilia in the Main Olfactory System of Mice
Published on: December 27, 2014
An endocannabinoid system is present in the mouse olfactory epithelium but does not modulate olfaction
C R Hutch1, C J Hillard2, C Jia3
1Neuroscience Program, Michigan State University, 293 Farm Lane, East Lansing, MI 48824, USA; Environmental and Integrative Toxicological Sciences, Michigan State University, 1129 Farm Lane, East Lansing, MI 48824, USA.
The endocannabinoid system, including cannabinoid receptors (CB1 and CB2), is present in the mouse olfactory epithelium. However, it does not appear to affect olfaction-mediated behaviors in adult mice.
Area of Science:
- Neuroscience
- Olfactory System Biology
- Endocannabinoid Research
Background:
- Endocannabinoids regulate various functions, including progenitor cell proliferation and olfaction.
- Their role in the mammalian peripheral olfactory epithelium remains unexamined.
Purpose of the Study:
- To investigate the presence and function of the endocannabinoid system in the mouse olfactory epithelium.
- To determine if endocannabinoids modulate olfactory sensory neuron populations and behavior.
Main Methods:
- Immunohistochemistry and calcium imaging to localize cannabinoid receptors (CB1/CB2).
- Analysis of olfactory sensory neuron and basal cell numbers in wildtype and CB1/CB2 receptor-deficient mice.
- Behavioral testing using the buried food and habituation/dishabituation tests.
Main Results:
- CB1 and CB2 receptor protein and mRNA were detected in the olfactory epithelium.
- CB1 receptors were localized on neurons, sustentacular cells, microvillous cells, and basal cells.
- CB1(-/-)/CB2(-/-) mice showed a slight decrease in basal cells and olfactory sensory neurons, but no behavioral deficits in olfaction.
Conclusions:
- An endocannabinoid system exists in the mouse olfactory epithelium.
- Endocannabinoids do not appear to influence adult olfactory function or behavior in mice.
- Further research is needed on the role of endocannabinoids in olfactory progenitor cell development.
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