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Related Experiment Video

Updated: Apr 11, 2026

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
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An efficient genetic manipulation protocol for Ustilago esculenta.

Jiajia Yu1, Yafen Zhang1, Haifeng Cui1

  • 1Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China.

FEMS Microbiology Letters
|June 4, 2015
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to genetically modify Ustilago esculenta, a fungus used in food. This breakthrough enables further study of the fungus-plant relationship and its unique edible galls.

Keywords:
Ustilago esculentaZizania latifoliaprotoplasttransformation

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Area of Science:

  • Mycology
  • Plant Pathology
  • Biotechnology

Background:

  • Ustilago esculenta forms edible galls on Zizania latifolia, a delicacy in China.
  • Understanding the U. esculenta-Z. latifolia interaction requires efficient genetic tools.
  • Current genetic manipulation methods for U. esculenta are limited.

Purpose of the Study:

  • To establish a robust protoplast-based transformation system for Ustilago esculenta.
  • To optimize conditions for high-yield protoplast production and transformation efficiency.
  • To demonstrate the utility of the system for gene disruption and overexpression.

Main Methods:

  • Optimized enzyme digestion (lywallzyme), osmotic stabilizers (sucrose), and temperature for protoplast isolation.
  • Tested various vectors and selection agents (hygromycin, carboxin resistance) for transformation.
  • Developed regeneration protocols on sucrose-containing media.

Main Results:

  • A haploid strain (UeT55) yielded optimal protoplasts with 15 mg/mL lywallzyme at 30°C for 3 hours.
  • Linear plasmids with hygromycin or carboxin resistance genes yielded higher transformation frequencies.
  • Successfully disrupted the UeICL gene and overexpressed the EGFP gene in U. esculenta.

Conclusions:

  • A reliable protoplast-based genetic transformation system for Ustilago esculenta has been established.
  • This system facilitates future research in forward and reverse genetics for this fungus.
  • The developed methods pave the way for deeper insights into the U. esculenta-Z. latifolia symbiosis.