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Updated: Apr 11, 2026
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Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
Published on: April 4, 2016
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Extrachromosomal circular DNA is common in yeast.
Henrik D Møller1, Lance Parsons2, Tue S Jørgensen1
1Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark;
Summary
Extrachromosomal circular DNAs (eccDNAs) are common in yeast and can significantly impact genetic variation. These circular DNA molecules, found across the genome, may play a key role in evolution.
Area of Science:
- Genetics
- Molecular Biology
- Genomics
Background:
- Extrachromosomal circular DNAs (eccDNAs) are prevalent in various organisms.
- The genome-wide impact of eccDNAs on genetic variation remains largely unexplored.
Purpose of the Study:
- To investigate the prevalence and characteristics of eccDNAs in the Saccharomyces cerevisiae genome.
- To assess the potential contribution of eccDNAs to genetic variation and evolution.
Main Methods:
- Utilized Circle-Seq, a sensitive eccDNA purification technique.
- Mapped 1,756 eccDNAs within the S. cerevisiae genome.
Main Results:
- Yeast eccDNAs range from 1 kb to 38 kb, encompassing 23% of the genome and thousands of genes.
- EccDNAs originate from both repetitive and non-repetitive genomic regions.
- Common eccDNAs include ribosomal genes, transposon remnants, and tandemly repeated genes; 80% contain replication origins.
Conclusions:
- EccDNAs are widespread in S. cerevisiae.
- These circular DNA elements likely contribute significantly to genetic variation and evolutionary processes.
Keywords:
DNA replication originschromosomal instabilitycircular DNAgene deletionhomologous recombinationMore Related Videos
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