A dispersive liquid-liquid microextraction and chiral separation of carvedilol in human plasma using capillary
Background:
Development of simple, rapid and precise analysis of chiral drugs in biological samples is an important issue. Dispersive liquid-liquid microextraction in combination with CE using field amplified sample injection has been of interest because of its capability to analyze trace amount of drugs.
Methods:
Dispersive liquid-liquid microextraction-CE-field amplified sample injection was employed for chiral separation of carvedilol in human plasma using UV-DAD detector and the developed method has been validated according to US FDA method validation guideline for bioanalysis.
Results:
The method was linear over a concentration range of 12.5-100 ng/ml for each carvedilol enantiomer (R(2) = 0.998) and the mean recoveries ranged from 91 to 107%.
Conclusion:
The method was adapted for sensitive, selective and rapid determination of carvedilol enantiomers in human plasma samples.
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