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Generation of Alginate Microspheres for Biomedical Applications
Published on: August 12, 2012
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Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D
Stefanie Utech1, Radivoje Prodanovic1,2, Angelo S Mao1
1School of Engineering and Applied Sciences, Harvard University, 02138, Cambridge, MA, USA.
Advanced Healthcare Materials
|June 4, 2015
Abstract:
Monodisperse alginate microgels (10-50 μm) are created via droplet-based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.
