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Related Experiment Video

Updated: Apr 11, 2026

De Novo Generation of Somatic Stem Cells by YAP/TAZ
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FACS Sorting Mammary Stem Cells.

Oihana Iriondo1, Miriam Rábano, María D M Vivanco

  • 1CIC bioGUNE, Technological Park of Bizkaia, Building 801A, 48160, Derio, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|June 5, 2015
PubMed
Summary
This summary is machine-generated.

Fluorescent-activated cell sorting (FACS) is a key method for isolating mammary gland stem cells and breast cancer stem cells. This protocol details FACS for breast cancer stem cell separation, with principles applicable to other cell types.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Oncology

Background:

  • Stem cells, including those in the mammary gland, are crucial for tissue development and regeneration.
  • Identifying and isolating specific stem cell populations, such as breast cancer stem cells, is essential for understanding tumor biology and developing targeted therapies.
  • Fluorescent-activated cell sorting (FACS) is a powerful technique for cell analysis and purification based on physical and chemical characteristics.

Purpose of the Study:

  • To describe a detailed protocol for using FACS to isolate and characterize breast cancer stem cells.
  • To highlight the utility of FACS in distinguishing stem cells from healthy mammary glands and malignant tumors.
  • To provide a generalizable methodology for applying FACS to various cell sorting applications.

Main Methods:

  • Utilizing FACS to separate breast cancer stem cells based on cell surface antigen recognition.
  • Employing a combination of approaches, including analysis of cellular activities, to identify stem cell populations.
  • Describing the step-by-step protocol for FACS-based cell sorting.

Main Results:

  • FACS enables the effective isolation of stem cells from both healthy mammary glands and breast tumors.
  • The described protocol facilitates the specific separation of breast cancer stem cells.
  • The general principles of the FACS protocol are applicable to the sorting of diverse cell types.

Conclusions:

  • FACS is an indispensable technique for the isolation and characterization of mammary gland and breast cancer stem cells.
  • The presented protocol offers a reliable method for breast cancer stem cell separation.
  • The adaptability of the FACS methodology extends its utility to a broader range of cell sorting applications in biological research.