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Real Time RT-PCR

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Related Experiment Video

Updated: Apr 11, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

Nucleic acid quantification using nicking-displacement, rolling circle amplification and bio-bar-code mediated

Xue-Mei Li¹, Jie Luo², Ning-Bo Zhang³

¹School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, PR China.

Analytica Chimica Acta

|June 5, 2015

Summary

A novel triple-amplification system using inductively-coupled plasma-mass spectrometry (ICP-MS) enables highly sensitive detection of hepatitis B virus (HBV) DNA. This method accurately identifies genetic variations in complex biological samples for biomedical research.

Keywords:

Bio-bar-code Hepatitis B virus Inductively-coupled plasma-mass spectrometry Rolling circle amplification Triple-amplification

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Last Updated: Apr 11, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

Amplicon Sequencing using the Long-Read Sequencing Technologies

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Area of Science:

Biotechnology
Analytical Chemistry
Molecular Diagnostics

Background:

Sensitive detection of specific DNA sequences is crucial for diagnosing diseases like hepatitis B.
Existing methods may lack the sensitivity or specificity required for early detection or complex sample analysis.
Advancements in amplification techniques are needed to improve DNA detection limits.

Purpose of the Study:

To develop and validate a highly sensitive DNA detection system.
To demonstrate the system's capability in detecting hepatitis B virus (HBV) DNA.
To assess the potential for discriminating single nucleotide polymorphisms (SNPs) and analyzing complex biological samples.

Main Methods:

Fabrication of an inductively-coupled plasma-mass spectrometry (ICP-MS)-based triple-amplification system.
Integration of nicking-displacement, rolling circle amplification (RCA), and bio-bar-code probes.
Utilizing DNA probes labeled with gold nanoparticles (AuNPs) for enhanced signal detection.

Main Results:

Achieved detection of hepatitis B virus (HBV) DNA down to 3.2×10⁻¹⁷ M.
Demonstrated effective discrimination of single nucleotide polymorphisms (SNPs).
Successfully detected target DNA in complicated biological samples, confirming robustness.

Conclusions:

The developed ICP-MS-based triple-amplification system offers exceptional sensitivity and specificity for DNA detection.
This strategy holds significant potential for sensitive diagnosis of viral infections like HBV.
The system is a promising tool for advanced biomedical research and clinical diagnostics.