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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Related Experiment Video

Updated: Apr 11, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

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Emerging fluorescent protein technologies.

Jhon Ralph Enterina1, Lanshi Wu1, Robert E Campbell1

  • 1Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada.

Current Opinion in Chemical Biology
|June 5, 2015
PubMed
Summary

Fluorescent proteins (FPs) are essential biological research tools. This review explores novel applications of FPs in advanced biosensing and gene expression manipulation for future biological studies.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Fluorescent proteins (FPs), exemplified by green fluorescent protein (GFP), are indispensable tools in biological research.
  • FPs function as genetically encoded markers for tracking cellular components and as a basis for biosensors.

Purpose of the Study:

  • To review emerging applications of fluorescent proteins.
  • To highlight innovative strategies in FP-based biosensing.
  • To explore novel uses of FPs for manipulating protein function and gene expression.

Main Methods:

  • Literature review of recent advancements in fluorescent protein technology.
  • Analysis of new methodologies employing FPs for biological research.
  • Synthesis of current trends and future directions in FP applications.

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Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

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Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast
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Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

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Main Results:

  • FPs offer versatile applications beyond traditional marking, including sophisticated biosensing.
  • Emerging strategies enable precise visualization of biochemical events.
  • Innovative techniques allow for the manipulation of protein function and gene expression using FPs.

Conclusions:

  • Fluorescent proteins are evolving into powerful tools for advanced biological research.
  • New FP applications promise to expand our understanding of cellular processes.
  • The field is moving towards dynamic control and sensing of biological systems using FPs.