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High sensitivity automated multiplexed immunoassays using photonic crystal enhanced fluorescence microfluidic system
Yafang Tan1, Tiantian Tang1, Haisheng Xu2
1Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 208 North Wright Street, Urbana, IL 61801, USA.
Biosensors & Bioelectronics
|June 5, 2015
Summary
This study presents a microfluidic platform using photonic crystal enhanced fluorescence (PCEF) for highly sensitive biomarker detection. The automated system achieves single-digit pg/mL sensitivity with low sample volumes for point-of-care diagnostics.
Area of Science:
- Biophotonics
- Microfluidics
- Assay Development
Background:
- Surface-based fluorescent assays require high sensitivity and selectivity.
- Microfluidic devices offer advantages in sample handling and automation.
- Photonic crystals can enhance fluorescence signals.
Purpose of the Study:
- To develop an integrated platform combining microfluidics and PCEF for sensitive biomarker detection.
- To achieve high analytic sensitivity, selectivity, low sample volume, and assay automation.
- To demonstrate the platform's utility for multiplexed biomarker analysis.
Main Methods:
- Integration of a photonic crystal (PC) surface with a microfluidic cartridge.
- Utilizing PC optical resonances to amplify Cyanine 5 (Cy5) fluorescence.
- Employing an automated fluid handling system for sequential reagent introduction.
- Performing biomarker assays for interleukin 3 (IL3) and Tumor Necrosis Factor (TNF-α).
Main Results:
- Achieved single-digit pg/mL limits of detection for IL3 and TNF-α.
- Demonstrated high analytic sensitivity and selectivity.
- Reduced spot-to-spot variability compared to manual assays.
- Confirmed compatibility with low sample volumes and assay automation.
Conclusions:
- The integrated PCEF and microfluidic platform enables highly sensitive and automated biomarker detection.
- The system offers significant improvements in assay precision and reproducibility.
- This technology is suitable for developing diagnostic instruments for point-of-care applications.
