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Updated: Apr 11, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Comparing different reactor configurations for Partial Nitritation/Anammox at low temperatures.
Eva M Gilbert1, Shelesh Agrawal1, Thomas Schwartz2
1Karlsruhe Institute of Technology, Engler-Bunte-Institute, Water Chemistry and Water Technology, Engler-Bunte-Ring 1, 76131, Karlsruhe, Germany.
Biomass geometry significantly impacts Partial Nitritation/Anammox (PN/A) performance in cold wastewater. Granular and biofilm PN/A systems maintain activity down to 13°C, unlike suspended biomass.
Area of Science:
- Environmental Engineering
- Microbiology
- Wastewater Treatment
Background:
- Partial Nitritation/Anammox (PN/A) is effective for nitrogen removal from warm, concentrated wastewater.
- Current research focuses on applying PN/A to cooler, dilute municipal wastewater.
- Low temperatures (10-20°C) present a significant challenge for PN/A processes.
Purpose of the Study:
- To investigate the impact of temperature reduction on PN/A performance.
- To compare the behavior of different biomass configurations under realistic temperature gradients.
- To identify optimal biomass geometries for low-temperature PN/A.
Main Methods:
- Four lab-scale reactors with different biomass configurations (suspended, granular, biofilm) were operated.
- A simulated seasonal temperature gradient from 20°C down to 10°C was applied.
- Synthetic wastewater was used to isolate PN/A process behavior.
Main Results:
- Anammox activity in suspended biomass declined significantly at 15°C.
- Granular biomass and biofilms on carriers maintained anammox activity down to <13°C.
- Thicker biofilms showed greater resilience and adaptation to low temperatures compared to thinner biofilms.
Conclusions:
- Biomass geometry is critical for PN/A efficiency in cold conditions.
- Granular and biofilm carriers are superior to suspended biomass for low-temperature PN/A.
- Biofilm thickness influences resilience and adaptation to temperature fluctuations in PN/A systems.
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