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Related Concept Videos

RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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RNA Splicing01:32

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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RNA Structure01:23

RNA Structure

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Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
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RNA Structure01:19

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The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
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Related Experiment Video

Updated: Apr 11, 2026

A Nonsequencing Approach for the Rapid Detection of RNA Editing
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A Nonsequencing Approach for the Rapid Detection of RNA Editing

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RCARE: RNA Sequence Comparison and Annotation for RNA Editing.

Soo Youn Lee, Je-Gun Joung, Chan Hee Park

    BMC Medical Genomics
    |June 6, 2015
    PubMed
    Summary

    This study introduces RCARE, a new tool for identifying RNA editing sites. RCARE enhances the accuracy of RNA editing detection and annotation, distinguishing true sites from false positives.

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    Area of Science:

    • Molecular Biology
    • Bioinformatics
    • Genomics

    Background:

    • RNA editing is a crucial post-transcriptional modification regulating protein function and linked to human diseases.
    • Accurate identification of RNA editing sites (RNA-DNA differences or RDDs) is essential but challenged by numerous false positives.
    • Distinguishing true RDD sites and understanding condition-specific editing is vital for biological insights.

    Purpose of the Study:

    • To develop a robust tool, RCARE (RNA-sequence comparison and annotation for RNA editing), for searching, annotating, and visualizing RDD sites.
    • To improve the reliability of identified RDD sites through evidence-based annotation.
    • To enable comparative analyses of RNA editing across multiple samples.

    Main Methods:

    • RCARE was developed as a web-based platform for RDD site analysis.
    • It utilizes a comprehensive database of known editing sites for comparative analysis.
    • A Python script facilitates conversion of alignment files to VCF for upload, and the tool provides evidence levels for reliability assessment.

    Main Results:

    • RCARE enables efficient searching, annotation, and visualization of RDD sites.
    • The tool provides rich biological annotations and summary plots for user-friendly interpretation.
    • RCARE generates evidence levels, addressing the lack of reliability metrics in previous tools.

    Conclusions:

    • RCARE offers an improved method for identifying and annotating RNA editing sites, enhancing reliability.
    • The tool aids in distinguishing true RNA editing events from false positives.
    • RCARE is a valuable resource for researchers studying RNA editing and its role in biological processes and diseases.