Related Experiment Video
Updated: Apr 11, 2026

05:59
Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology
Published on: September 26, 2020
6.7K
Blotting from PhastGel to Membranes by Ultrasound
1Department of Chemical Engineering, Ben Gurion University of the Negev, Beer-Sheva, 84105, Israel, kost@bgu.ac.il.
Methods in Molecular Biology (Clifton, N.J.)
|June 6, 2015
Abstract:
Ultrasound based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm(2)) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500-106,000 Da) and 14C-labeled Rainbow protein molecular weight markers (14,300-200,000 Da).

