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Related Concept Videos

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
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The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Related Experiment Video

Updated: Apr 11, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
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Resolution pattern for mass spectrometry imaging.

Stephan R Fagerer1, Andreas Römpp2, Konstantins Jefimovs3

  • 1ETH Zürich, Department of Chemistry and Applied Biosciences, 8093, Zürich, Switzerland.

Rapid Communications in Mass Spectrometry : RCM
|June 6, 2015
PubMed
Summary

Researchers developed microstructured patterns for determining mass spectrometry imaging (MSI) resolution. This provides a standard method for comparing MSI instruments and quality control, achieving resolutions around 50 µm.

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Area of Science:

  • Analytical Chemistry
  • Imaging Science

Background:

  • Mass spectrometry imaging (MSI) lacks a standardized method for assessing spatial resolution.
  • A universal standard is needed for comparing MSI setups across laboratories and for quality control.

Purpose of the Study:

  • To develop and validate microstructured resolution patterns for determining MSI spatial resolution.
  • To establish a convenient and reliable method for MSI instrument performance evaluation.

Main Methods:

  • Fabrication of microstructured resolution patterns via laser machining into metal foils.
  • Creation of patterns with hydrophilic grooves on omniphobic surfaces for sample handling.
  • Testing patterns on a commercial matrix-assisted laser desorption/ionization (MALDI) imaging instrument.

Main Results:

  • Demonstrated capability to determine spatial resolution down to a few micrometers.
  • Achieved a spatial resolution of approximately 50 µm on a MALDI imaging instrument.
  • Confirmed compatibility of pattern features with high-resolution MALDI imaging systems.

Conclusions:

  • Metal resolution grids and patterned glass slides are effective for determining MALDI imaging hardware resolution.
  • These methods offer a straightforward approach for both production and application.
  • The developed patterns facilitate convenient and reliable MSI resolution assessment.