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Sealing capability of implant-abutment junction under cyclic loading: a toluidine blue in vitro study
Antonio Scarano1, Vittoria Perrotti1, Adriano Piattelli1
1Department of Medical, Oral and Biotechnological Sciences, University G. d'Annunzio of Chieti-Pescara, Chieti - Italy.
Purpose:
The aim of the present in vitro study was to evaluate the leakage observed in external hexagon (EH) and cone Morse (CM) tapered implant-abutment connections, using toluidine blue.
Methods:
A total of 60 implants, 30 with a screw-retained EH abutment and 30 with a CM taper internal connection, were used. Toluidine blue was placed into the deepest portion of the internal compartment of the 2 different implant systems, and cyclic loading was applied for each group as follows: 10 samples underwent 1 × 10(6) loading cycles, 10 samples underwent 3 × 10(6) cyclic loading and the least 10 samples underwent 6 × 10(6) cyclic loading.
Results:
No significant differences between the EH and CM groups were detected when the lowest loading cycles were applied (p = 0.2624), while differences were found when the samples were loaded with 3 x 10(6) and 6 x 10(6) cycles (p = 0.00124), with significantly lower toluidine leakage in CM group.
Conclusions:
In conclusion, the results of the present in vitro study demonstrated that flow of the toluidine blue to the external portion of the implant-abutment assembly occurred in both types of implant-abutment connections, with very different percentages. Indeed, the CM taper internal connection seems to be more resistant to the leakage of dyes when compared with EH connections.

