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Related Experiment Videos

Enucleation for treating rodent ocular disease.

Laura A Wilding1, Mayu Uchihashi2, Ingrid L Bergin2

  • 1Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, Michigan, USA. laurawildingdvm@gmail.com.

Journal of the American Association for Laboratory Animal Science : JAALAS
|June 6, 2015
PubMed
Summary

Enucleation surgery offers a successful alternative to euthanasia for rodents with severe corneal disease unresponsive to medical treatment. This refined procedure preserves valuable research animals and reduces premature study termination.

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Area of Science:

  • Veterinary Surgery
  • Ophthalmology
  • Rodent Models

Background:

  • Current standard care for rodent corneal lesions involves medical management, often leading to euthanasia when unsuccessful.
  • Euthanasia results in premature loss of valuable genetically modified and long-term study rodents.

Purpose of the Study:

  • To investigate enucleation surgery as a treatment for rodent corneal disease unresponsive to medical management.
  • To assess the efficacy and safety of enucleation as a refinement to the standard of care.

Main Methods:

  • Enucleation performed under isoflurane anesthesia, including globe removal, hemostasis, orbital packing with absorbable gelatin sponge, and tarsorrhaphy.
  • Postoperative analgesia provided with buprenorphine and carprofen.
  • Histologic examination at 1 and 3 months post-surgery.

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Main Results:

  • 100% success rate in 15 clinically affected rodents and 5 healthy controls with a 60-day follow-up.
  • Single complication of tarsorrhaphy dehiscence, successfully repaired.
  • Histology showed no evidence of infection at the enucleation site.

Conclusions:

  • Rodent enucleation is a straightforward surgical refinement to the standard of care.
  • The procedure does not cause significant inflammation of periocular structures.
  • Enucleation reduces euthanasia rates for rodents with severe ocular lesions, preserving study integrity.