Related Experiment Video
Updated: Apr 11, 2026
Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo
Published on: February 6, 2015
ING4 enhances paclitaxel's effect on colorectal cancer growth in vitro and in vivo
Liyu Cao1, Shunhua Chen2, Cong Zhang2
1Department of Gastroenterology, The First Affiliated Hospital, Anhui Medical University Hefei 230022, Anhui Province, China ; Department of Pathology, Anhui Medical University Hefei 230032, Anhui Province, China.
Abstract:
Inhibitor of growth 4 (ING4) is a tumor suppressor that can inhibit cell growth and induce apoptosis. ING4 expression levels show negative correlation with the clinical stage, histological grade, and lymph node metastasis of colorectal cancer. Further insights are needed to analyze the effect of adenovirus-mediated ING4 on colorectal cancer cell growth and the response to paclitaxel treatment. In this study, we found adenovirus-mediated ING4 expression reduced proliferation and enhanced apoptosis in the SW1116 cells. p-Stat3 and Ki-67 expression significantly decreased in the SW1116 cells treated with Ad-ING4, PTX, or Ad-ING4+PTX compared with those treated with PBS or Ad-GFP both in vitro and in vivo (P<0.05). In animal experiments, the mice treated with Ad-ING4, PTX, or Ad-ING4+PTX exhibited significantly inhibited growth of SW1116 xenografts compared with those treated with PBS or Ad-GFP (P<0.05) and the combination (Ad-ING4+PTX) treatment exhibited the highest inhibition. Our results highlight that Ad-ING4 significantly inhibits growth and induces apoptosis in SW1116 colorectal cancer cells and suppresses tumor growth in SW1116 xenografts by downregulating p-Stat3 and Ki-67 expression. A combination of Ad-ING4 and PTX exhibits the highest inhibition, indicating that ING4 enhances sensitivity to chemotherapy.
Insights
Inhibitor of growth 4 (ING4) delivered via adenovirus suppresses colorectal cancer cell growth and induces apoptosis. Combining ING4 with paclitaxel (PTX) shows the greatest tumor inhibition, enhancing chemotherapy sensitivity.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Inhibitor of growth 4 (ING4) acts as a tumor suppressor, inhibiting cell proliferation and inducing apoptosis.
- ING4 expression correlates inversely with colorectal cancer stage, grade, and metastasis.
- The role of adenovirus-mediated ING4 in colorectal cancer and its interaction with paclitaxel requires further investigation.
Purpose of the Study:
- To investigate the effect of adenovirus-mediated ING4 on colorectal cancer cell growth.
- To evaluate the combined effect of ING4 and paclitaxel on colorectal cancer.
- To analyze the molecular mechanisms underlying ING4's action in colorectal cancer.
Main Methods:
- Adenovirus-mediated ING4 (Ad-ING4) was used to treat SW1116 colorectal cancer cells in vitro and in vivo.
- Cell proliferation and apoptosis were assessed.
- Expression levels of p-Stat3 and Ki-67 were measured.
- Tumor growth in SW1116 xenografts was monitored.
Main Results:
- Ad-ING4 significantly reduced proliferation and enhanced apoptosis in SW1116 cells.
- Ad-ING4, paclitaxel (PTX), and combination therapy (Ad-ING4+PTX) downregulated p-Stat3 and Ki-67 expression.
- In vivo, Ad-ING4, PTX, and combination therapy inhibited SW1116 xenograft growth, with combination therapy showing the highest efficacy.
- ING4 enhanced sensitivity to paclitaxel chemotherapy.
Conclusions:
- Adenovirus-mediated ING4 inhibits colorectal cancer cell growth and induces apoptosis.
- ING4 suppresses tumor growth by downregulating p-Stat3 and Ki-67.
- Combination therapy with Ad-ING4 and paclitaxel demonstrates significant therapeutic potential for colorectal cancer.
More Related Videos
09:29Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
Published on: September 30, 2016
06:49Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer
Published on: February 10, 2023
