Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Improving Translational Accuracy02:07

Improving Translational Accuracy

3.8K
3.8K
Leaky Scanning02:28

Leaky Scanning

5.9K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Profile of bacterial meningitis pathogens circulating in a cohort of school children in Côte d'Ivoire using 16S rRNA gene sequencing.

BMC infectious diseases·2026
Same author

Airway immune signatures of protection and disease progression in recent human tuberculosis household contacts.

Nature immunology·2026
Same author

Efferocytosis of apoptotic bodies drives SARS-CoV-2 infection and macrophage inflammation.

Nature communications·2026
Same author

An update on recent developments in the drug treatment of tuberculous meningitis.

Expert opinion on pharmacotherapy·2026
Same author

Predicting trajectories of illness using RNA velocity of whole blood.

Nature communications·2026
Same author

Contribution of cytotoxic CD8 T cells, neutrophils and type 1 interferon signaling to hyperinflammation in HIV-associated TB meningitis.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Apr 11, 2026

IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae
08:22

IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae

Published on: January 15, 2020

6.7K

Tricks to translating TB transcriptomics.

Armin Deffur1, Robert J Wilkinson1, Anna K Coussens1

  • 11 Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, 2 Department of Medicine, Faculty of Health Sciences, University of Cape Town and Groote Schuur Hospital, Observatory, 7925, South Africa ; 3 The Francis Crick Institute, Mill Hill Laboratory, London, NW7 1AA, UK ; 4 Department of Medicine, Imperial College London, W2 1PG, UK.

Annals of Translational Medicine
|June 6, 2015
PubMed
Summary
This summary is machine-generated.

Researchers identified IL-32 as a key mediator in tuberculosis (TB) protection, linking interferon gamma (IFNγ) and vitamin D. This finding offers potential for new TB diagnostics and therapeutics.

Keywords:
Biomarkercorrelates of riskmicroarrayprotective immunitysystems biologytuberculosis (TB)

More Related Videos

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
07:42

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

Published on: June 5, 2014

8.5K
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

8.3K

Related Experiment Videos

Last Updated: Apr 11, 2026

IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae
08:22

IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae

Published on: January 15, 2020

6.7K
A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
07:42

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

Published on: June 5, 2014

8.5K
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

8.3K

Area of Science:

  • Tuberculosis (TB) pathogenesis research.
  • Systems biology and transcriptomics applications.
  • Immunology and infectious disease research.

Background:

  • High-throughput transcriptomics is crucial for understanding tuberculosis (TB) pathogenesis.
  • Previous studies identified correlates of TB risk and late-stage disease.
  • Identifying correlates of protection is vital for developing new TB diagnostics and therapeutics.

Purpose of the Study:

  • To identify correlates of protection against TB using complex analysis of transcriptomic data.
  • To explore the role of IL-32 as a mediator of antimicrobial immunity and a marker of latent TB infection (LTBI).
  • To review existing TB transcriptomic studies and discuss challenges in data analysis and interpretation.

Main Methods:

  • Complex data analysis of existing TB transcriptomic datasets.
  • Utilizing in vitro models and latent TB infection (LTBI) as a surrogate for protection.
  • Reviewing all TB whole-blood transcriptomic studies and analyzing weighted gene co-expression network analysis (WGCNA).

Main Results:

  • IL-32 identified as a mediator of interferon gamma (IFNγ)-vitamin D dependent antimicrobial immunity.
  • IL-32 found to be a marker of latent TB infection (LTBI).
  • Discussion of potential pitfalls in combining transcriptomic analyses and the importance of metadata.

Conclusions:

  • IL-32 is a potential biomarker for TB protection.
  • Integrated OMICS platforms and open access to metadata are proposed for TB research.
  • Leveraging OMICS data is essential for discovering TB correlates of protection.