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Plant Tissue Culture

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

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Related Experiment Video

Updated: Apr 11, 2026

Asymbiotic Germination and Leaf Explant-Based Regeneration of the Endangered Medicinal Orchid Hemipilia cucullata from Mature Seeds

Asymbiotic Germination and Leaf Explant-Based Regeneration of the Endangered Medicinal Orchid Hemipilia cucullata from Mature Seeds

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Dendrobium micropropagation: a review.

Jaime A Teixeira da Silva, Jean Carlos Cardoso, Judit Dobránszki

Plant Cell Reports

|June 6, 2015

Summary

This summary is machine-generated.

This review details Dendrobium orchid micropropagation techniques, summarizing half a decade of research. It offers optimized protocols for efficient mass propagation and germplasm conservation.

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Last Updated: Apr 11, 2026

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Published on: September 19, 2025

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Area of Science:

Plant Biotechnology
Horticulture
Orchidology

Background:

Dendrobium orchids are significant for ornamental and medicinal uses.
Micropropagation via tissue culture is a key method for Dendrobium propagation.

Purpose of the Study:

To provide a comprehensive review of Dendrobium micropropagation literature.
To enable efficient protocol development and optimization for Dendrobium germplasm.

Main Methods:

Chronological analysis of published Dendrobium micropropagation studies.
Detailed examination of explants, media, growth regulators, and culture conditions.

Main Results:

Compilation of optimized parameters for Dendrobium in vitro culture.
Identification of key factors influencing organogenesis and plantlet development.

Conclusions:

This review facilitates improved mass propagation and conservation of Dendrobium orchids.
It supports advanced breeding techniques like polyploidization and genetic transformation using tissue culture.