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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Pre-mRNA Processing: RNA Splicing

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

RNA Stability

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Related Experiment Video

Updated: Apr 11, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

SnapShot: Spliceosome Dynamics I.

Markus C Wahl¹, Reinhard Lührmann²

¹Laboratory of Structural Biochemistry, Freie Universität Berlin, Takustraße 6, 14195 Berlin, Germany.

|June 6, 2015

Summary

This summary is machine-generated.

Spliceosomes are dynamic molecular machines that perform pre-messenger RNA (mRNA) splicing. These complex structures assemble on-demand for each splicing event, removing introns and joining exons for protein synthesis.

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Last Updated: Apr 11, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

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Published on: June 30, 2022

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Published on: September 15, 2021

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

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Published on: November 9, 2020

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Spliceosomes are large RNA-protein complexes essential for gene expression in eukaryotes.
They mediate pre-mRNA splicing, a critical step in producing mature messenger RNA (mRNA).
Splicing involves removing non-coding introns and joining coding exons.

Purpose of the Study:

To elucidate the dynamic nature and assembly process of spliceosomes.
To understand the fundamental mechanisms of pre-mRNA splicing.
To provide insights into the function of these molecular machines in protein biosynthesis.

Main Methods:

The study focuses on the structural and functional aspects of spliceosomes.
It examines the stepwise recruitment of subunits during assembly.
Analysis of RNA-protein interactions and conformational changes.

Main Results:

Spliceosomes are demonstrated to be highly dynamic molecular machines.
They assemble 'de novo' for each splicing cycle.
The process involves the sequential addition of multiple subunits onto the pre-mRNA substrate.

Conclusions:

Spliceosomes represent a paradigm of dynamic molecular machinery.
Their assembly and function are crucial for accurate gene expression.
Understanding spliceosome dynamics is key to comprehending eukaryotic gene regulation.