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Related Concept Videos

Proteomics01:33

Proteomics

10.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.2K

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Related Experiment Video

Updated: Apr 11, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

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An effective plasma membrane proteomics approach for small tissue samples.

Katrien Smolders1, Nathalie Lombaert1, Dirk Valkenborg2

  • 1KU Leuven, Department of Biology, Laboratory of Neuroplasticity and Neuroproteomics, Leuven, Belgium.

Scientific Reports
|June 6, 2015
PubMed
Summary

Researchers developed a new method to study plasma membrane proteins in small tissue samples. This technique identifies over 1,600 proteins, aiding the search for novel drug targets.

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Last Updated: Apr 11, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

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A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
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A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

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Area of Science:

  • Proteomics
  • Cell Biology
  • Drug Discovery

Background:

  • Identifying new drug targets is crucial for advancing medicine.
  • Studying the plasma membrane proteome is key to understanding cell function and disease.
  • Existing methods are often not suitable for small or functionally specific tissue samples.

Purpose of the Study:

  • To develop and validate an innovative research strategy for plasma membrane proteome analysis.
  • To enable the study of proteomes in small, functionally defined tissue samples.
  • To facilitate the discovery of novel drug targets through comprehensive proteomic analysis.

Main Methods:

  • Biotinylation of acute tissue slices to label surface proteins.
  • Streptavidin pull-down assay for selective protein enrichment.
  • Shotgun proteomics for high-throughput protein identification and quantification.

Main Results:

  • Successfully extracted and identified over 1,600 unique proteins from millimetre-cubed scale tissue samples.
  • Demonstrated that over 60% of identified proteins are associated with the plasma membrane.
  • Identified key plasma membrane proteins including G-protein coupled receptors, ion channels, and transporters.

Conclusions:

  • The developed biotinylation and proteomics strategy is effective for analyzing plasma membrane proteomes in small tissue samples.
  • This approach significantly advances the capability to discover novel drug targets by providing a detailed view of cell surface proteins.
  • The method's applicability to minute tissue volumes opens new avenues for research in various biological and medical fields.