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Optimization of the determination of tocopherols in Agaricus sp. edible mushrooms by a normal phase liquid
Lillian Barros1, Daniela M Correia2, Isabel C F R Ferreira2
1CIMO-Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, Apartado 1172, 5301-855 Bragança, Portugal; Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Campus Miguel de Unamuno s/n, E-37007 Salamanca, Spain.
Abstract:
Individual tocopherol profile of five Agaricus mushroom species, widely consumed in Portugal, was obtained by a normal-phase high-performance liquid chromatography (NP-HPLC). It was used a simple solid-liquid extraction procedure without saponification step and the chromatographic separation was achieved using a YMC-Pack Polyamine II column using an isocratic elution with hexane/ethyl acetate (70:30, v/v) at a flow rate of 1.0mL/min. The effluent was monitored by a fluorescence detector. All the compounds were separated in a period of time of 30min. The method proved to be sensitive, reproducible and accurate, allowing the determination of tocopherols.
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