Related Experiment Video
Updated: Apr 11, 2026
GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
Determination of biogenic diamines with a vaporisation derivatisation approach using solid-phase microextraction gas
M Ali Awan1, I Fleet2, C L Paul Thomas3
1Institute of Environmental Science and Engineering, National University of Sciences and Technology, Rawalpindi, Pakistan.
Abstract:
A gas-phase on-fibre derivatisation method for the determination of putrescine and cadaverine by gas chromatography/mass spectrometry using trifluoroacetylacetone (TFAA) has been studied and optimised. Small amounts (2μl) of putrescine, cadaverine and TFAA standards were vaporised at high temperature in a 20cm(3) closed SPME vial. The subsequent derivatives were recovered from the headspace of the vial using a PDMS/DVB fibre. The optimised mole ratio for [TFAA]/[Putrescine+Cadaverine] reaction was 22.3/1 with a derivatisation and extraction temperature of 120(o)C and an extraction time of 20min. The retention times for the derivatised putrescine and cadaverine were 20.5 and 22.2min, respectively on a capillary column, CP-Sil 8CB; 30m length×0.25mmi.d.×0.25μm film thickness. The correlation coefficients (R(2)) of calibration curves for putrescine and cadaverine were 0.999 and 0.997, respectively over a range of sample masses of 20-350ng, using nonadecane as an internal standard. Putrescine and cadaverine recoveries were determined to be 93.9% and 103.3%, respectively. The method was found to be a straightforward single step procedure that was unaffected by complex sample matrices and was successfully tested on samples of meat, vegetables and cheese.
More Related Videos
12:47Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
Published on: January 22, 2018
04:19Author Spotlight: An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry
Published on: March 10, 2023
Related Concept Videos
01:19Gas Chromatography: Types of Detectors-II
01:14Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
00:55Mass Spectrometry: Amine Fragmentation
In amines, the number of nitrogen atoms affects the mass of the molecular ion, which is described by the nitrogen rule of mass spectrometry. This rule states that a compound containing a single...
01:13Gas Chromatography: Introduction
In GC, a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a...