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Updated: Apr 11, 2026

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
Published on: May 13, 2016
Hepassocin is required for hepatic outgrowth during zebrafish hepatogenesis
Ming Gao1, Hui Yan2, Rong-Hua Yin3
1Tianjin University, Department of Pharmaceutical Engineering, Tianjin 300072, China; Beijing Institute of Radiation Medicine, Beijing 100850, China.
Background & Aims:
Hepassocin (HPS) is a hepatotrophic growth factor that specifically stimulates hepatocyte proliferation and promotes liver regeneration after liver damage. In this paper, zebrafish were used to investigate the role of HPS in liver development.
Methods And Results:
During zebrafish development, HPS expression is enriched in liver throughout hepatogenesis. Knockdown of HPS using its specific morpholino leads to a smaller liver phenotype. Further results showed that the HPS knockdown has no effect on the expression of the early endoderm marker gata6 and early hepatic marker hhex. In addition, results showed that the smaller-liver phenotype in HPS morphants was caused by suppression of cell proliferation, not induction of cell apoptosis.
Conclusions:
Current findings indicated that HPS is essential to the later stages of development in vertebrate liver organogenesis.
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