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Related Concept Videos

Immunofluorescence Microscopy01:12

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Immunogold Electron Microscopy01:20

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
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Immunofluorescence vs immunochemiluminescent methods: AIA 2000 vs Immulite 2000.

Chiara Cosma1, Silvia Baggio1, Alessandra Casarotti1

  • 1Department of Laboratory Medicine, University-Hospital, Padua, Italy.

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|June 7, 2015
PubMed
Summary

This study validates the Tosoh AIA 2000 automated system for measuring C-peptide, insulin, sex hormone binding globulin (SHBG), and dehydroepiandrosterone sulfate (DHEAS). The system shows reliable analytical performance, comparable to established methods for these specialized hormone tests.

Keywords:
C-peptideDHEA-SInsulinSHBG

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Area of Science:

  • Clinical Chemistry
  • Endocrinology
  • Laboratory Medicine

Background:

  • Specific hormone measurements like C-peptide, insulin, SHBG, and DHEAS are crucial for diagnosing various endocrine disorders.
  • These specialized tests are not always available in routine clinical laboratories, necessitating reliable automated systems.
  • Evaluating new automated platforms is essential for improving diagnostic capabilities and accessibility.

Purpose of the Study:

  • To assess the analytical performance of the Tosoh AIA 2000 automated system for measuring C-peptide, insulin, sex hormone binding globulin (SHBG), and dehydroepiandrosterone sulfate (DHEAS).
  • To compare the performance of the AIA 2000 system with existing widely used chemiluminescent methods.

Main Methods:

  • The Tosoh AIA 2000 automated system was utilized for the quantitative measurement of C-peptide, insulin, SHBG, and DHEAS.
  • Analytical performance characteristics of the AIA 2000 were evaluated.
  • Results were compared with data from an established chemiluminescent system (Immulite 2000).

Main Results:

  • The Tosoh AIA 2000 system demonstrated satisfactory analytical performance for all evaluated hormone assays.
  • The fluorescence detection system of the AIA 2000 provided performance comparable to the chemiluminescent Immulite 2000 system.
  • Excellent comparability was observed between the AIA 2000 and the reference method for these frequently underutilized clinical hormones.

Conclusions:

  • The Tosoh AIA 2000 automated system is a reliable platform for the measurement of C-peptide, insulin, SHBG, and DHEAS.
  • The system's performance supports its use in clinical laboratories for specialized hormone testing.
  • The findings suggest the AIA 2000 can provide accurate and comparable results to established methods, enhancing diagnostic utility.