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Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
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Ribonuclease-neutralized pancreatic microsomal membranes from livestock for in vitro co-translational protein

Kurt Vermeire1, Susanne Allan2, Becky Provinciael1

  • 1Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, KU Leuven-University of Leuven, B-3000 Leuven, Belgium.

Analytical Biochemistry
|June 9, 2015
PubMed

Keywords:
Cell-free protein translationCyclotriazadisulfonamide (CADA)Mammalian ER membranesPancreatic microsomesRibonucleaseTranslocation inhibitor

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