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EDTA: Chemistry and Properties01:22

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Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
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The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
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Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
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Complexation Equilibria: The Chelate Effect01:19

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In complexation reactions, metal atoms or cations interact with ligands to form donor-acceptor adducts called metal complexes. Ligands that bind through one donor site are monodentate, ligands with two donor sites are bidentate, and those with more than two donor sites are polydentate ligands. For example, ethylene diamine is a bidentate ligand that binds through two nitrogen donor atoms, forming a five-membered ring. EDTA is a polydentate ligand that binds through four oxygen and two nitrogen...
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EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
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The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
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Metalated nucleotide chemisorption on hydroxyapatite.

Michele Benedetti1, Daniela Antonucci1, Federica De Castro1

  • 1University of Salento, Department of Biological and Environmental Sciences and Technologies, Via Monteroni, 73100, Lecce, Italy.

Journal of Inorganic Biochemistry
|June 9, 2015
PubMed
Summary

The net negative charge of nucleotide derivatives is crucial for adsorption onto hydroxyapatite nanocrystals (nHAP). Phosphate presence alone does not ensure binding; charge dictates chemisorption efficiency for bone targeting and drug delivery applications.

Keywords:
Antitumor drugAntiviral drugHydroxyapatite nanocrystalsNanomaterialsNucleotide adsorptionPlatinum complex

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Biochemistry

Background:

  • Hydroxyapatite nanocrystals (nHAP) are key components in bone tissue and drug delivery systems.
  • Understanding the adsorption of nucleotide derivatives onto nHAP is vital for developing targeted therapies.
  • The role of molecular charge in adsorption processes requires further elucidation.

Purpose of the Study:

  • To investigate the influence of residual charge on nucleotide derivatives for adsorption onto nHAP in aqueous solutions.
  • To determine the specific charge requirements for effective chemisorption on nHAP.
  • To provide insights for modeling the binding of phosphate-bearing molecules to hydroxyapatite.

Main Methods:

  • Experimental investigation of nucleotide derivative adsorption on nHAP.
  • Analysis of adsorption based on the net charge of the nucleotide derivative (cationic, neutral, negative).
  • Evaluation of the role of phosphate groups in modulating adsorption efficiency.

Main Results:

  • The presence of phosphates alone does not guarantee adsorption onto nHAP.
  • Cationic or neutral charges on nucleotide derivatives significantly reduce chemisorption.
  • A net negative charge on nucleotide derivatives results in relevant adsorption onto nHAP.
  • Phosphate number modulates adsorption only if the molecule is negatively charged.
  • Neutral zwitterionic nucleotide complexes with phosphates showed no stable chemisorption.

Conclusions:

  • Net negative charge is the primary determinant for nucleotide derivative adsorption on nHAP.
  • Charge, not just phosphate presence, governs chemisorption, impacting potential applications.
  • Findings are crucial for designing nHAP-mediated drug delivery and bone tissue targeting strategies.