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Platelet membrane abnormalities in myeloproliferative disorders: decrease in glycoproteins Ib and IIb/IIIa complex is

M Mazzucato1, L De Marco, V De Angelis

  • 1Centro Immunotrasfusionale e Chimica Clinica, C.R.O. Aviano, U.S.L. n. 11, Pordenone, Italy.

British Journal of Haematology
|November 1, 1989
PubMed
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Platelet membrane glycoproteins (GP) Ib and IIb/IIIa are often abnormal in myeloproliferative disorders (MPD). These changes correlate with reduced function and may explain bleeding risks in MPD patients.

Area of Science:

  • Hematology
  • Oncology
  • Biochemistry

Background:

  • Myeloproliferative disorders (MPD) are a group of conditions affecting blood cell production.
  • Platelets play a crucial role in hemostasis, and their membrane glycoproteins are key to function.
  • Abnormalities in platelet glycoproteins have been implicated in bleeding tendencies in MPD.

Purpose of the Study:

  • To investigate the number and functional activity of platelet membrane glycoproteins (GP) Ib and IIb/IIIa in patients with MPD.
  • To determine if alterations in these glycoproteins correlate with clinical manifestations like bleeding.

Main Methods:

  • Quantification of GPIb and GPIIb/IIIa on platelet surfaces using 125I-labelled monoclonal antibodies.
  • Assessment of GPIb and IIb/IIIa functional activity via binding assays with labeled asialo von Willebrand factor (vWF) and fibrinogen.

Related Experiment Videos

  • Scatchard analysis to evaluate receptor binding affinity.

    • Main Results:

    • Decreased binding of anti-GPIIb/IIIa antibody (approx. 40%) and reduced fibrinogen binding to activated platelets in most MPD patients.
    • Slightly decreased or unchanged binding of anti-GPIb antibodies in most patients, correlating with reduced asialo vWF binding.
    • Normal receptor binding affinity observed in Scatchard analysis for most ligands and patients.
    • Increased plasma glycocalicin levels were dependent on high platelet counts.

    Conclusions:

    • Abnormalities in the concentration and function of GPIIb/IIIa are common in MPD patients.
    • Decreased GPIb concentration and function are also observed in a subset of MPD patients.
    • These glycoprotein abnormalities may contribute to the hemorrhagic tendency frequently seen in MPD.