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Three novel oligosaccharides with the sialyl-Lea structure in human milk: isolation by immunoaffinity chromatography
H Kitagawa1, H Nakada, A Kurosaka
1Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Abstract:
We have determined the structures of three novel oligosaccharides isolated from human milk using the monoclonal antibody MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography. From the results of 500-MHz 1H NMR spectroscopy and fast atom bombardment-mass spectrometry, their structures were deduced to be (formula; see text) These oligosaccharides bound to MSW 113 to nearly the same extent as sialyl-Lea hexasaccharide but bound to another sialyl-Lea structure-directed monoclonal antibody, NS 19-9, only weakly.