[Subfractionated conversions of high density lipoproteins in vitro in sera with various lipid spectra]

Using gradient gel electrophoresis, the interconversions of high density lipoproteins (HDL) upon heating for 24 hours at 37 degrees C of blood sera obtained from dyslipidemic patients, were studied. It was shown that during incubation of normolipidemic, hypercholesterolemic and hyperalphacholesterolemic sera, the portion of large particles of HDL2 subclass (HDL2b) is increased with a simultaneous decrease in the portion of the smallest particles of HDL3 subclass (HDL3b and HDL3c). Contrariwise, in hypertriglyceridemic and hypoalphacholesterolemic sera the portion of the largest HDL2b particles is decreased, while that of middle-sized HDL3a is increased. Hence, hypertriglyceridemia and hypoalphacholesterolemia are not associated with an intensive conversion of small HDL3 particles into large HDL2 ones, which may be the reason for the decreased cholesterol transport function of HDL.