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lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
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The STAT3-regulated long non-coding RNA Lethe promote the HCV replication.

Yulin Xiong1, Jing Yuan1, Changjiang Zhang1

  • 1Institute of Infectious Diseases of Chinese PLA, Southwest Hospital, Third Military Medical University, Gaotanyan Centre Street No. 30, Shapingba district, Chongqing 400038, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|June 10, 2015
PubMed
Summary
This summary is machine-generated.

Long non-coding RNA Lethe, regulated by STAT3, promotes Hepatitis C virus (HCV) replication. Knockdown of Lethe partially blocked HCV replication and upregulated interferon-stimulated genes (ISGs).

Keywords:
HCV replicationLetheLong non-coding RNAsSTAT3

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Area of Science:

  • Molecular Biology
  • Virology
  • RNA Biology

Background:

  • Long non-coding RNAs (lncRNAs) are crucial in biological processes but their role in Hepatitis C Virus (HCV) replication is understudied.
  • STAT3 signaling is implicated in viral infections, but its interaction with lncRNAs in HCV pathogenesis requires further investigation.

Purpose of the Study:

  • To investigate the role of lncRNAs regulated by phosphorylated STAT3 in HCV replication.
  • To identify specific lncRNAs involved in HCV replication and elucidate their regulatory mechanisms.

Main Methods:

  • Utilized human PCR array to identify lncRNAs modulated by phosphorylated STAT3.
  • Employed siRNA-mediated knockdown of lnc-Lethe in Huh7 cells to assess its impact on HCV replication.
  • Quantified expression levels of interferon-stimulated genes (ISGs) including PKR, OAS, and IRF1 post Lethe knockdown.

Main Results:

  • Phosphorylated STAT3 was found to promote HCV replication.
  • lnc-Lethe was identified as a key lncRNA involved in HCV replication, with its knockdown partially inhibiting viral replication.
  • Lethe knockdown led to the upregulation of ISGs (PKR, OAS, IRF1).

Conclusions:

  • lnc-Lethe, upregulated by activated STAT3, promotes HCV replication.
  • Lethe may negatively regulate the type I Interferon (IFN) response, thereby facilitating HCV replication.
  • These findings highlight a novel regulatory pathway involving lncRNAs in HCV pathogenesis.