Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biofuels01:25

Biofuels

84
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...
84
Bioreactor Controls-III01:22

Bioreactor Controls-III

54
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
54
Production of Organic Acids01:25

Production of Organic Acids

80
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
80
Fates of Pyruvate01:20

Fates of Pyruvate

12.3K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
12.3K
Hydroboration-Oxidation of Alkenes03:08

Hydroboration-Oxidation of Alkenes

12.5K
In addition to the oxymercuration–demercuration method, which converts the alkenes to alcohols with Markovnikov orientation, a complementary hydroboration-oxidation method yields the anti-Markovnikov product. The hydroboration reaction, discovered in 1959 by H.C. Brown, involves the addition of a B–H bond of borane to an alkene giving an organoborane intermediate. The oxidation of this intermediate with basic hydrogen peroxide forms an alcohol.
12.5K
Upstream Processing01:27

Upstream Processing

84
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
84

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A metabolic model based on a pangenome core reveals putative conserved biochemical features of the phytopathogen Xylella fastidiosa.

Microbiological research·2026
Same author

An Anti-Inflammatory Signature Across Pain and Cognition: Not All Mediterranean Diets Are Equal.

Nutrients·2026
Same author

Incorporation of Cryptic Plasmid Energetics Improves Genome-Scale Metabolic Predictions in Probiotic E. coli Nissle 1917.

Microbial biotechnology·2026
Same author

<i>Bifidobacterium animalis</i> subsp. <i>lactis</i> CECT 8145 BPL1<sup>®</sup> Laxative Effects in Loperamide-Induced Constipated SD Rats.

Nutrients·2026
Same author

Engineering <i>Pseudomonas putida</i> for the Production of 2,3-Butanediol Isomers and Acetoin.

ACS synthetic biology·2026
Same author

Genetic engineering of gut commensals: heterologous protein expression in <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> CECT8145.

Current research in microbial sciences·2026

Related Experiment Video

Updated: Apr 11, 2026

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
09:10

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

21.5K

Engineering alternative isobutanol production platforms.

Carmen Felpeto-Santero1, Antonia Rojas, Marta Tortajada

  • 1Department of Environmental Biology, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain, cfelpetosantero@cib.csic.es.

AMB Express
|June 10, 2015
PubMed
Summary

Researchers engineered a synthetic inducible operon (IbPSO) for isobutanol production. This system enables new microbial strains to efficiently produce isobutanol from various carbon sources, including biomass.

More Related Videos

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

15.4K
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

12.0K

Related Experiment Videos

Last Updated: Apr 11, 2026

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
09:10

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

21.5K
Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

15.4K
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

12.0K

Area of Science:

  • Synthetic biology
  • Metabolic engineering
  • Microbial biotechnology

Background:

  • Developing efficient microbial platforms for biofuel production is crucial for sustainable energy.
  • Current methods for isobutanol synthesis often face challenges with yield and feedstock flexibility.

Purpose of the Study:

  • To design and construct a synthetic inducible operon (IbPSO) for isobutanol production.
  • To create and test recombinant microbial strains capable of producing isobutanol from diverse carbon sources.

Main Methods:

  • Designed a synthetic inducible operon (IbPSO) encoding genes for the conversion of pyruvate to 2-isobutyraldehyde.
  • Constructed two recombinant plasmids (pIZIbPSO and p424IbPSO) carrying the IbPSO.
  • Transformed wild-type bacteria (Shimwellia blattae and Escherichia coli W) with the plasmids.
  • Assessed isobutanol production using glucose, sucrose, and hydrolysed lignocellulosic biomass as carbon sources.

Main Results:

  • Shimwellia blattae harboring p424IbPSO produced up to 6 g/L of isobutanol from glucose.
  • Escherichia coli W transformed with pIZIbPSO demonstrated isobutanol production from sucrose for the first time.
  • Recombinant strains successfully produced isobutanol from raw carbon sources like lignocellulosic biomass hydrolysates.

Conclusions:

  • The synthetic inducible operon (IbPSO) is effective for creating robust isobutanol-producing microbial strains.
  • This system offers a versatile platform for testing bacterial hosts and optimizing isobutanol production from various feedstocks.
  • The demonstrated ability to utilize lignocellulosic biomass highlights the potential for sustainable and cost-effective isobutanol biofuel production.