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Updated: Apr 11, 2026

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
Relative Strengths of Promoters Provided by Common Mobile Genetic Elements Associated with Resistance Gene Expression
Muhammad Kamruzzaman1, Jason D Patterson1, Shereen Shoma1
1Centre for Infectious Diseases and Microbiology, The Westmead Millennium Institute for Medical Research, The University of Sydney, Westmead Hospital, Westmead, New South Wales, Australia.
Abstract:
Comparison of green fluorescent protein expression from outward-facing promoters (POUT) of ISAba1, ISEcp1, and ISAba125 revealed approximate equivalence in strength, intermediate between PCS (strong) and PCWTGN-10 (weak) class 1 integron promoter variants, >30-fold stronger than POUT of ISCR1, and >5 times stronger than Ptac. Consistent with its usual role, PCWTGN-10 produces more mRNA from a "downstream" gfp gene transcriptionally linked to a "usual" PCWTGN-10-associated gene cassette than does POUT of ISAba1.
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