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Regulation by a chaperone improves substrate selectivity during cotranslational protein targeting.

Aileen Ariosa1, Jae Ho Lee1, Shuai Wang1

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125.

Proceedings of the National Academy of Sciences of the United States of America
|June 10, 2015
PubMed
Summary

Trigger factor (TF) controls signal recognition particle (SRP) function, ensuring nascent polypeptides are correctly sorted for protein biogenesis. This regulation at the ribosome exit site enhances protein folding and localization accuracy.

Keywords:
GTPasesprotein biogenesisribosomesignal recognition particletrigger factor

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Area of Science:

  • Molecular Biology
  • Protein Biogenesis
  • Cellular Mechanisms

Background:

  • The ribosome exit site is a crowded cellular compartment.
  • Nascent polypeptides interact with various factors for proper folding, localization, and quality control.
  • Accurate sorting of polypeptides into specific pathways is crucial for protein biogenesis.

Purpose of the Study:

  • To investigate the mechanism by which nascent polypeptides are sorted between trigger factor (TF) and signal recognition particle (SRP).
  • To understand how TF regulates SRP function at the ribosome exit site.

Main Methods:

  • Investigated the interplay between TF and SRP at the ribosome exit site.
  • Probed the regulation of SRP function by TF.

Main Results:

  • TF regulates SRP function at three key stages: ribosome binding, membrane targeting, and polypeptide rejection.
  • TF enhances the specificity of substrate selection for both TF and SRP pathways.
  • Demonstrated a multilayered mechanism of molecular interaction at the ribosome exit site.

Conclusions:

  • TF plays a critical role in regulating SRP activity, ensuring proper protein targeting and biogenesis.
  • The findings provide a framework for understanding protein sorting in the crowded ribosome exit site environment.
  • This molecular interplay ensures proteins are directed to the correct biogenesis machineries.