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Effect of Bacterial Lipopolysaccharide Contamination on Gutta Percha- versus Resilon-Induced Human Monocyte Cell Line
Jamshid Hadjati1, Hadi Assadian2, Abdollah Ghorbanzadeh3
1Associate Professor, Department of Immunology, Medical School, Tehran University of Medical Sciences and Health Services, Tehran, Iran.
Objectives:
Cytotoxic effects of obturation materials were tested in presence and absence of endotoxin on human monocytes in vitro.
Materials And Methods:
Human monocytes from THP-1 cell line were cultured. Three millimeters from the tip of each Resilon and gutta percha points were cut and directly placed at the bottom of the culture wells. Cultured cells were exposed to gutta percha (groups G1 and G2) and Resilon (R1 and R2). Ten μg/ml bacterial lipopolysaccharide (LPS) was added to the culture wells in groups G1 and R1. Positive control included the bacterial LPS without the root canal filling material and the negative control contained the cells in culture medium only. Viability of cells was tested in all groups after 24, 48, and 72 hours using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay for at least 3 times to obtain reproducible results. Optical density values were read and the data were analyzed using three-way ANOVA and post hoc statistical test.
Results:
The results showed that cells in G2 had the lowest rate of viability at 24 hours, but the lowest rate of viable cells was recorded in G1 at 48 and 72 hours. The effect of LPS treatment was not statistically significant. Resilon groups showed cell viability values higher than those of gutta percha groups, although statistically non-significant (P=0.105). Cell viability values were lower in gutta percha than Resilon groups when LPS-treated and LPS-untreated groups were compared independently at each time point.
Conclusion:
It could be concluded that none of the tested root canal filling materials had toxic effects on cultured human monocyte cells whether in presence or absence of LPS contamination.
Insights
This study found that neither Resilon nor gutta percha root canal filling materials showed toxic effects on human monocytes, even with bacterial endotoxin (LPS) contamination.
Area of Science:
- Biomaterials Science
- Dental Materials
- Cell Biology
Background:
- Root canal obturation materials are crucial for endodontic treatment success.
- Assessing the biocompatibility of these materials is essential for patient safety.
- Previous studies have indicated varying cytotoxic potentials of different obturation materials.
Purpose of the Study:
- To evaluate the in vitro cytotoxic effects of Resilon and gutta percha on human monocytes.
- To determine if bacterial endotoxin (lipopolysaccharide - LPS) influences the cytotoxicity of these materials.
Main Methods:
- Human monocyte cell line (THP-1) was cultured.
- Resilon and gutta percha samples were placed in direct contact with monocytes.
- Cells were exposed to bacterial lipopolysaccharide (LPS) in specific groups.
- Cell viability was assessed using the MTT assay at 24, 48, and 72 hours.
Main Results:
- Gutta percha groups exhibited lower cell viability compared to Resilon groups, though not statistically significant.
- The presence of LPS did not significantly alter the cytotoxic effects of the materials.
- Overall, no tested root canal filling material demonstrated significant toxicity to human monocytes.
Conclusions:
- Resilon and gutta percha are biocompatible with human monocytes in vitro.
- The materials do not exhibit significant cytotoxic effects, even in the presence of bacterial endotoxin.
- These findings support the safety of using Resilon and gutta percha in endodontic procedures.
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