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Related Experiment Video

Updated: Apr 11, 2026

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

Dark dyes-bright complexes: fluorogenic protein labeling.

Marcel P Bruchez¹

¹Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA; Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA; Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.

Current Opinion in Chemical Biology

|June 10, 2015

Summary

Researchers developed a new method for brighter, more detectable fluorescent labeling using organic dyes and genetically encoded proteins. This approach simplifies biological assays and improves labeling in living organisms.

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Area of Science:

Biochemistry
Molecular Biology
Biotechnology

Background:

Organic dyes and genetically encoded proteins offer stable, tunable, and targetable fluorescent labeling.
Challenges exist in achieving bright fluorescence and avoiding unbound dye removal.

Purpose of the Study:

To develop an optimized approach for controlling dye properties for enhanced fluorescent labeling.
To enable simpler, more robust biological assays in various model systems.

Main Methods:

Utilizing chemical or environmental changes to modulate dye fluorescence.
Developing complexes of organic dyes with genetically encoded proteins.

Main Results:

Achieved conversion of weakly fluorescent dyes to bright, easily detectable complexes.
Eliminated the need for unbound dye removal.

Conclusions:

Optimized dye-protein complexes offer robust and simplified fluorescent labeling.
This method facilitates rapid assays in cultured cells and improves hybrid labeling in living organisms.