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Updated: Apr 11, 2026

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Published on: January 5, 2017

DATASW, a tool for HPLC-SAXS data analysis.

Alexander V Shkumatov¹, Sergei V Strelkov¹

¹Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Herestraat 49, 3000 Leuven, Belgium.

Acta Crystallographica. Section D, Biological Crystallography

|June 10, 2015

Summary

This summary is machine-generated.

This study introduces DATASW, a program for analyzing heterogeneous protein samples using high-performance liquid chromatography coupled with small-angle X-ray scattering (HPLC-SAXS). DATASW averages data frames to determine structural parameters and predict folding states.

Keywords:

HPLC SAXS oligomer processing

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Last Updated: Apr 11, 2026

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Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

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Published on: November 5, 2018

Area of Science:

Structural biology
Biophysical techniques

Background:

Small-angle X-ray scattering (SAXS) is a valuable low-resolution technique for structural analysis.
Sample monodispersity is crucial for accurate SAXS data interpretation and structural modeling.
High-performance liquid chromatography (HPLC) coupled with SAXS (HPLC-SAXS) enables the analysis of heterogeneous samples.

Purpose of the Study:

To present DATASW, a software tool for processing HPLC-SAXS data.
To enable accurate structural parameter determination and folding state prediction for protein samples.

Main Methods:

Development of the DATASW program for data analysis.
Implementation of a sliding window approach for averaging individual data frames from HPLC-SAXS experiments.
Calculation of key SAXS parameters: I(0), Rg, Dmax, and molecular weight.

Main Results:

DATASW effectively averages data from HPLC-SAXS experiments.
The software calculates essential structural parameters and predicts protein folding states.
Demonstrated utility across various proteins with diverse oligomerization behaviors on different beamlines.

Conclusions:

DATASW is a useful tool for analyzing complex protein samples using HPLC-SAXS.
The program aids in reliable interpretation of SAXS data for heterogeneous systems.
Software is available for download for broad accessibility in structural biology research.