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Does tube diameter influence the packed cell volume?

M A Karlow1, J C Westengard, B S Bull

  • 1Loma Linda University, CA 92350.

Clinical and Laboratory Haematology
|January 1, 1989
PubMed
Summary
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Smaller packed cell volume (PCV) tube diameters lead to decreased PCV readings due to red blood cell dehydration. Glass type influences dehydration, with soda-lime glass causing more errors than borosilicate glass.

Area of Science:

  • Hematology
  • Clinical Pathology
  • Biomedical Engineering

Background:

  • Accurate packed cell volume (PCV) determination is crucial for diagnosing various medical conditions.
  • Previous studies have not fully elucidated the impact of PCV tube dimensions and material on PCV measurements.
  • Potential sources of error, such as red blood cell dehydration and trapped plasma, require further investigation.

Purpose of the Study:

  • To investigate the relationship between observed PCV and PCV tube diameter.
  • To develop a predictive equation for PCV based on PCV tube radius and glass type.
  • To quantify red blood cell dehydration during centrifugation and its correlation with glass material.

Main Methods:

  • PCV determinations were performed on five patient blood samples using eight tubes of varying diameters.

Related Experiment Videos

  • Tubes were made of either soda-lime glass or borosilicate glass.
  • Red blood cell dehydration was assessed by counting spheroechinocytes in the bottom of the PCV tubes.

    • Main Results:

    • PCV decreased as PCV tube diameter decreased.
    • A derived equation accurately predicted PCV as a function of tube radius.
    • Soda-lime glass tubes exhibited twice the red blood cell dehydration compared to borosilicate glass tubes.

    Conclusions:

    • Red blood cell dehydration during high-speed centrifugation is influenced by PCV tube diameter and glass type.
    • Dehydration errors, particularly with soda-lime glass, must be considered alongside trapped plasma errors for accurate PCV determination.
    • The findings necessitate careful selection of PCV tubes for reliable reference PCV measurements.