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Hydrogen sulfide mediated cascade reaction forming an iminocoumarin: applications in fluorescent probe development
Pratryush Kumar Mishra1, Tanmoy Saha, Pinaki Talukdar
1Department of Chemistry, Indian Institute of Science Education and Research Pune, India. ptalukdar@iiserpune.ac.in.
Abstract:
The study on a fluorescent probe that undergoes a H2S mediated cascade reaction to form an iminocoumarin fluorophore is reported. The probe features better water solubility and fast sensing time (t1/2 = 6.1 min and response time = 24 min) as key advances compared to the reported probe that works on a similar mechanism. The sensing mechanism of the probe was demonstrated by mass spectrometric, HPLC titration and FT-IR titration methods. H2S sensing by the probe was characterized by a 31-fold fluorescence enhancement and alimit of detection of 169 nM. Application of the probe was demonstrated by imaging of H2S in live cells.
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