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Updated: Apr 11, 2026

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

Tracking single-particle rotation during macrophage uptake.

Lucero Sanchez¹, Paul Patton, Stephen M Anthony

¹Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA. yy33@indiana.edu.

|June 11, 2015

Summary

This summary is machine-generated.

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Single microparticles show varied rotation speeds during macrophage cell uptake. Particle size and surface ligands minimally impacted this complex rotational behavior during internalization.

Area of Science:

Biophysics
Cell Biology
Materials Science

Background:

Cellular internalization processes are crucial for biological functions.
Understanding microparticle dynamics during cellular uptake informs drug delivery and biomaterial design.
Macrophage cells play a key role in immune responses and particle clearance.

Purpose of the Study:

To investigate the rotational dynamics of single microparticles during macrophage cell internalization.
To quantify particle orientation and rotation using optical anisotropy.
To determine the influence of particle size and surface ligands on rotational behavior.

Main Methods:

Utilized triblock patchy microparticles with two fluorescent poles for visualization.
Employed optical anisotropy to track and quantify particle orientation and rotation in real-time.

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Last Updated: Apr 11, 2026

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Published on: October 19, 2018

Time-lapse Imaging of Mouse Macrophage Chemotaxis

Time-lapse Imaging of Mouse Macrophage Chemotaxis

Published on: April 2, 2020

Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1

Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1

Published on: September 5, 2016

Observed microparticle internalization by macrophage cells.

Main Results:

Microparticles exhibited a heterogeneous mix of fast and slow rotation during macrophage uptake.
Particles demonstrated transient directional rotation upon entering the cell.
Particle size and surface ligand presentation had a negligible effect on rotational heterogeneity.

Conclusions:

Microparticle rotation during macrophage internalization is complex and varied.
The observed rotational dynamics are largely independent of particle size and surface ligands.
These findings contribute to the understanding of particle-cell interactions and intracellular transport mechanisms.