Monoclonal antibodies in ABO serology: an evaluation

Monoclonal anti-A and anti-B reagents produced from mouse 'hybridoma' cells have been evaluated by comparative and standard serological techniques and found suitable for use as potent routine blood grouping reagents. They have an avidity time of only 9-12 seconds and their reactions are pH dependent with optimal reactions around neutral pH. The antisera gave good agglutination reactions at room temperature. The cost effectiveness and advantages of monoclonal antibodies include their high potency and the possibility of their production in unlimited amounts from in-vitro cultures.