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Related Experiment Video

Updated: Apr 11, 2026

In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model
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A Simple Optical Coherence Tomography Quantification Method for Choroidal Neovascularization.

Rania S Sulaiman1,2,3,4, Judith Quigley1,2, Xiaoping Qi1,2

  • 11 Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine , Indianapolis, Indiana.

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
|June 11, 2015
PubMed
Summary

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Optical coherence tomography (OCT) ellipsoid volume measurements accurately assess laser-induced choroidal neovascularization (L-CNV) lesion size in rodents. This in vivo method offers a reliable alternative for monitoring therapeutic efficacy in preclinical models.

Area of Science:

  • Ophthalmology
  • Preclinical Research
  • Medical Imaging

Background:

  • Laser-induced choroidal neovascularization (L-CNV) rodent models are standard for assessing therapeutic efficacy.
  • Current methods rely on ex vivo lesion size measurements using confocal microscopy.
  • A need exists for efficient, in vivo quantitative assessment methods.

Purpose of the Study:

  • To evaluate optical coherence tomography (OCT) ellipsoid volume measurements as a quantitative method for assessing L-CNV.
  • To compare OCT-based quantification with standard ex vivo confocal microscopy.
  • To determine if OCT can monitor treatment-induced changes in L-CNV lesions.

Main Methods:

  • Inducing L-CNV in C57BL/6J mice via argon laser and intravitreal injections (anti-VEGF164 or vehicle).

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  • Acquiring in vivo OCT images at multiple time points post-laser.
  • Calculating neovascular lesion volume as an ellipsoid from OCT and comparing with ex vivo confocal microscopy data.

    • Main Results:

    • OCT ellipsoid volume measurements showed strong correlation with ex vivo lesion volumes (Spearman's ρ=0.82-0.82).
    • The method enabled temporal monitoring of CNV lesions.
    • Evaluation of anti-VEGF treatment effects was feasible using this approach.

    Conclusions:

    • OCT ellipsoid volume measurement provides rapid, quantitative in vivo assessment of CNV lesions.
    • This method is compatible with different OCT systems and sensitive to treatment variations.
    • It serves as a valuable adjunct to ex vivo methods for preclinical CNV studies and other ocular diseases.