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Updated: Oct 13, 2026

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
Published on: January 7, 2013
The protein kinase C inhibitor H-7 inhibits concanavalin A induced T-lymphocyte activation
1Chinese Medicinal Material Research Centre, Chinese University of Hong Kong, Shatin.
Abstract:
1. 1-(5-isoquinoline sulfonyl)-2-methylpiperazine (H-7), a protein kinase C inhibitor, was found to inhibit con A stimulated [3H]thymidine incorporation and cytosolic protein kinase C (PKC) activation in T-lymphocytes of mouse spleen. 2. The inhibitory effect of H-7 was both concentration and time-dependent. 3. H-7 exerted no inhibition when T-lymphocytes have been preincubated with con A for 10 hours or longer. 4. These results support the notion that PKC is an important element of the con A mitogenic signal transduction mechanism and the PKC signal is completed within the first 10 hr of con A incubation.
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