Diurnal questing behavior of Amblyomma mixtum (Acari: Ixodidae)

The diurnal questing behavior of Amblyomma mixtum Koch was monitored in the laboratory while held on a 12:12 diurnal cycle, and outdoors, using actographic chambers to determine the daily incidences of questing for a host. Nymphs and adults increased their questing activity during the morning. Adult activity peaked in the morning and continued until afternoon, whereas nymphal activity peaked in the afternoon. Nymphs and adults returned to the bottom of the chambers at night. The questing pattern for nymphs held outdoors was similar to that observed indoors. Both nymphs and adults had a higher average questing height when held at 95% RH than at 56% RH. The increase in average questing height was found to be due to fewer ticks questing-and for adults, a decrease in the mean questing height-at lower RH.