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Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats

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Published on: November 8, 2018

Cell dialysis by sharp electrodes can cause nonphysiological changes in neuron properties.

Scott L Hooper¹, Jeffrey B Thuma², Christoph Guschlbauer¹

¹Department of Biological Sciences, Ohio University, Athens, Ohio; and Zoologisches Institut, Universität zu Köln, Köln, Germany.

Journal of Neurophysiology

|June 12, 2015

Summary

Sharp electrode impalements can alter neuron properties, impacting electrophysiological recordings. Using cytoplasm-matched solutions minimizes these time-dependent variations for accurate neuron analysis.

Keywords:

current injection leech neuron properties stomatogastric

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Area of Science:

Neuroscience
Electrophysiology

Background:

Sharp electrode recordings are crucial for studying neuron electrophysiology.
Electrode fill solutions can potentially influence recorded neuron properties.

Purpose of the Study:

To investigate how different sharp electrode fill solutions affect neuron properties over time.
To determine the optimal electrode fill solutions for accurate electrophysiological measurements.

Main Methods:

Recorded from lobster and leech neurons using sharp electrodes filled with various solutions (e.g., KAc, K2SO4) with differing molarities.
Measured membrane potential, input resistance, and ion channel currents at regular intervals post-penetration.
Assessed cell size changes and confirmed ion leakage from electrodes.

Main Results:

Neuron properties varied significantly based on electrode fill solution molarity and time after penetration.
High molarity fills (≥2.5 M) induced substantial, time-dependent changes in neuron properties, including cell swelling in leeches.
Ion leakage from electrodes was confirmed, suggesting injected ions contribute to observed property changes.

Conclusions:

Sharp electrode impalement and ion injection can artifactually alter neuron electrophysiology.
Using electrode fill solutions that approximate cytoplasm ion concentrations is recommended for accurate sharp electrode recordings.
Careful selection of electrode fill solutions is critical to minimize experimental artifacts in electrophysiology.