Hydrocarbon emulsification by Candida tropicalis and Debaryomyces polymorphus

Potentiality of C. tropicalis and D. polymorphus, to produce surface active compounds (bioemulsifiers/biosurfactants) during shake cultivation on hexadecane and oily waste was studied. Better emulsification activity, specific towards aromatic hydrocarbons, was observed with C. tropicalis culture broth. Emulsification activity of culture broth was quite stable and was unaffected by change in pH and by increasing the concentration of NaCl up to 5%. The activity was marginally affected by heating in boiling water bath for 15 min, but inhibited to the tune of 90% by 0.3% CaCl2. The isolated bioemulsifying factor contained 40, 22 and 17.5% lipid, protein and carbohydrate, respectively.