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A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

Published on: February 11, 2019

Optimizing cell viability in droplet-based cell deposition.

Jan Hendriks¹, Claas Willem Visser², Sieger Henke¹

¹Department of Developmental BioEngineering, MIRA institute for Biomedical Technology &Technical Medicine, Faculty of Science and Technology, University of Twente, The Netherlands.

Scientific Reports

|June 13, 2015

Summary

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This summary is machine-generated.

This study introduces a model to predict cell viability after droplet impact in biofabrication. Understanding droplet size, velocity, and substrate properties enhances cell survival in methods like cell spraying.

Area of Science:

Biotechnology and Biomedical Engineering
Cell Biology
Materials Science

Background:

Cell viability after droplet impact is critical for biofabrication techniques like cell spraying and inkjet bioprinting.
Current understanding of cell survival mechanisms during droplet impact is limited, hindering technological advancement.

Purpose of the Study:

To develop an analytical model predicting cell viability based on droplet characteristics and impact dynamics.
To provide a framework for optimizing droplet-based cell deposition technologies.

Main Methods:

Developed a model linking cell survival to membrane elongation, influenced by droplet size, velocity, and substrate properties.
Validated the model using cell viability measurements from cell spraying experiments.

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A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Title Cell Encapsulation by Droplets

Title Cell Encapsulation by Droplets

Published on: October 1, 2007

Related Experiment Videos

Last Updated: Apr 10, 2026

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

Published on: February 11, 2019

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Title Cell Encapsulation by Droplets

Title Cell Encapsulation by Droplets

Published on: October 1, 2007

Main Results:

The model accurately predicts cell viability as a function of cell membrane elongation and droplet impact parameters.
Identified key droplet and substrate characteristics influencing cell survival during impact.

Conclusions:

The developed analytical model offers a tool for rational optimization of biofabrication techniques involving cell droplet deposition.
Practical recommendations are provided to improve cell viability in cell spraying for applications such as wound healing.