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Microbiome of the Eye01:22

Microbiome of the Eye

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The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...
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Quercetin and Resveratrol Decrease the Inflammatory and Oxidative Responses in Human Ocular Surface Epithelial Cells.

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Quercetin and resveratrol demonstrate anti-inflammatory and antioxidant properties in human ocular surface cells. These findings suggest potential therapeutic applications for inflammatory eye conditions.

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Pharmacology

Background:

  • Inflammatory and oxidative stress are implicated in ocular surface diseases.
  • Polyphenols like quercetin (QCT) and resveratrol (RES) are known for their antioxidant and anti-inflammatory properties.
  • Investigating their effects on ocular cells is crucial for understanding potential therapeutic benefits.

Purpose of the Study:

  • To evaluate the anti-inflammatory and antioxidant effects of quercetin (QCT) and resveratrol (RES) on human conjunctival (IOBA-NHC) and corneal (HCE) epithelial cells.
  • To determine the impact of QCT and RES, individually and in combination, on cytokine production and reactive oxygen species (ROS) generation.
  • To explore the therapeutic potential of these polyphenols in managing ocular surface inflammation.

Main Methods:

  • Human conjunctival (IOBA-NHC) and corneal (HCE) epithelial cell lines were treated with varying concentrations of QCT and RES, as well as a combined QCT+RES mixture.
  • Cells were stimulated with tumor necrosis factor-alpha (TNF-α) to induce inflammation or ultraviolet B (UV-B) radiation to induce oxidative stress.
  • Cytokine levels (IL-6, IL-8, IP-10, VEGF) and intracellular ROS production were quantified using immune bead-based arrays and a H2DCF-DA dye assay, respectively.

Main Results:

  • TNF-α stimulation increased pro-inflammatory cytokine secretion (IL-6, IL-8, IP-10) in both cell lines.
  • QCT and RES significantly reduced IL-6 and IP-10 secretion in a dose-dependent manner.
  • Both compounds, particularly QCT, demonstrated dose-dependent inhibition of IL-8 secretion.
  • UV-B radiation significantly elevated ROS production, which was dose-dependently reduced by QCT and RES in HCE cells, and by RES in IOBA-NHC cells.

Conclusions:

  • Quercetin and resveratrol exhibit significant anti-inflammatory and antioxidant effects on human conjunctival and corneal epithelial cells in vitro.
  • These polyphenols effectively modulate key inflammatory mediators and reduce oxidative stress markers.
  • The findings support the potential therapeutic utility of QCT and RES for treating inflammatory ocular surface diseases.