Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardiovascular toxicity as a globally harmonized system hazard trait: The evidence and the path forward.

Regulatory toxicology and pharmacology : RTP·2026
Same author

Validation of the Electrophilic Allergen Screening Assay for Detection of Key Event 1 of the Skin Sensitization Adverse Outcome Pathway.

Toxics·2026
Same author

Host-virus determinants of Ebola virus persistence in a human cerebral organoid model.

Nature microbiology·2026
Same author

QSAR in the AI Era: Reflections for Advancing Chemical Safety Assessment.

Chemical research in toxicology·2026
Same author

Using effect biomarker thresholds in regulatory risk assessment: an OECD-based framework for assessing cumulative exposures to chemical mixtures.

Environment international·2026
Same author

Perspectives on variability of <i>in vivo</i> toxicology studies: considerations for next-generation toxicology.

Frontiers in toxicology·2026

Related Experiment Video

Updated: Apr 10, 2026

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays
08:00

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays

Published on: December 4, 2016

8.0K

Screening Chemicals for Estrogen Receptor Bioactivity Using a Computational Model.

Patience Browne1, Richard S Judson2, Warren M Casey3

  • 1†U.S. EPA, Office of Chemical Safety and Pollution Prevention, Washington, D.C. 20004, United States.

Environmental Science & Technology
|June 13, 2015
PubMed
Summary

The U.S. Environmental Protection Agency (EPA) validated new computational methods to screen for endocrine disruptors. These high-throughput screening assays and models accurately assess estrogen receptor bioactivity, offering an alternative to traditional testing.

More Related Videos

In Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR Toolbox
05:47

In Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR Toolbox

Published on: August 28, 2019

14.8K
Screening for Phytoestrogens using a Cell-based Estrogen Receptor &#946; Reporter Assay
06:07

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Published on: June 7, 2020

5.4K

Related Experiment Videos

Last Updated: Apr 10, 2026

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays
08:00

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays

Published on: December 4, 2016

8.0K
In Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR Toolbox
05:47

In Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR Toolbox

Published on: August 28, 2019

14.8K
Screening for Phytoestrogens using a Cell-based Estrogen Receptor &#946; Reporter Assay
06:07

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Published on: June 7, 2020

5.4K

Area of Science:

  • Environmental toxicology
  • Computational toxicology
  • Endocrine disruption assessment

Background:

  • The U.S. Environmental Protection Agency (EPA) is exploring advanced methods for endocrine bioactivity evaluation.
  • Traditional screening methods can be time-consuming and resource-intensive.

Purpose of the Study:

  • To validate high-throughput screening (HTS) assays and computational models for endocrine disruptor screening.
  • To demonstrate the robustness of these new tools for the Endocrine Disruptor Screening Program (EDSP).

Main Methods:

  • Integrated results from 18 estrogen receptor (ER) ToxCast HTS assays into a computational model.
  • Validated model performance using reference chemicals and existing EDSP Tier 1 assay results.
  • Assessed model's ability to distinguish true bioactivity from assay interference and cytotoxicity.

Main Results:

  • The ToxCast ER model achieved 86% to 93% accuracy compared to reference chemicals.
  • Model accurately predicted results of EDSP Tier 1 assays and uterotrophic studies (84% to 100% accuracy).
  • Successfully identified active and inactive chemicals and provided relative ER bioactivity measures.

Conclusions:

  • Validated high-throughput assays and the ToxCast ER model are robust for endocrine bioactivity screening.
  • These methods offer a rapid and accurate alternative for identifying potential endocrine-disrupting chemicals.
  • EPA now accepts ToxCast ER model data for 1812 chemicals as alternatives for EDSP Tier 1 ER assays.