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Related Experiment Video

Updated: Apr 10, 2026

Minimally-invasive Technique for Injection into Rat Optic Nerve

Minimally-invasive Technique for Injection into Rat Optic Nerve

Published on: May 19, 2015

Minimally-invasive Technique for Injection into Rat Optic Nerve.

Kateryna Raykova¹, Melina V Jones¹, Hwa Huang¹

¹Department of Neurology, Johns Hopkins University.

Journal of Visualized Experiments : Jove

|June 13, 2015

Summary

Researchers developed a minimally-invasive surgical technique for direct injection into the rat optic nerve. This method enables efficient delivery of therapeutics into the central nervous system for regeneration studies.

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Area of Science:

Neuroscience
Regenerative Medicine
Surgical Techniques

Background:

The rat optic nerve serves as a valuable model for studying central nervous system regeneration.
Current methods for accessing the optic nerve can be invasive, potentially limiting research applications.

Purpose of the Study:

To describe a novel, minimally-invasive surgical approach for direct injection into the rat optic nerve.
To establish a reproducible technique for delivering substances into the central nervous system via the optic nerve.

Main Methods:

A technique involving minor fascial dissection from the orbital ridge was employed.
A conjunctival traction suture was used to gently retract the eye, improving visualization and access.
Direct injection into the optic nerve was performed under visualization.

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Transconjunctival Approach for Injection into the Rat Optic Nerve

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Ocular Therapeutic Delivery and Advanced Tissue Retrieval in Adult Rats

Ocular Therapeutic Delivery and Advanced Tissue Retrieval in Adult Rats

Published on: May 23, 2025

Related Experiment Videos

Last Updated: Apr 10, 2026

Minimally-invasive Technique for Injection into Rat Optic Nerve

Minimally-invasive Technique for Injection into Rat Optic Nerve

Published on: May 19, 2015

Transconjunctival Approach for Injection into the Rat Optic Nerve

Transconjunctival Approach for Injection into the Rat Optic Nerve

Published on: April 4, 2025

Ocular Therapeutic Delivery and Advanced Tissue Retrieval in Adult Rats

Ocular Therapeutic Delivery and Advanced Tissue Retrieval in Adult Rats

Published on: May 23, 2025

Main Results:

The described surgical approach allows for minimally-invasive access to the rat optic nerve.
Successful injection of dyed beads into the optic nerve was demonstrated, confirming the technique's efficacy.
This method bypasses the need for bone removal, reducing surgical complexity.

Conclusions:

This minimally-invasive surgical technique provides a reliable method for direct optic nerve injection in rats.
The technique facilitates the delivery of agents for stem cell regeneration research within the central nervous system.
This approach offers a valuable tool for advancing optic nerve regeneration studies.